Three-dimensional cell geometry controls excitable membrane signaling in Dictyostelium cells
M. Hörning and T. Shibata
Biophysical Journal, 2019, (manuscript in press)
- doi : 10.1016/j.bpj.2018.12.012
(BioRxiv, 278853, 2018 - doi : 10.1101/278853)
Brief Summary: ''... Using a novel method of three-dimensional analysis of the entire cell membrane of Dictyostelium cells, we found that PtdInsP3 domains can propagate persistently in any direction on the entire plasma membrane, while their propagation direction and speed are governed by the geometry of the cell. This study opens more general questions about how the system geometry and spatiotemporal signaling interact to control pattern dynamics. ...''
Dynamics of spatiotemporal line defects and chaos control in complex excitable systems
M. Hörning*, F. Blanchard, A. Isomura and K. Yoshikawa
Scientific Reports, 7, 7757, 2017 - doi : 10.1038/s41598-017-08011-z
Brief Summary: ''... We show the spatiotemporal dynamics of line defects in rotating spiral waves. We combined a novel signaling over-sampling technique with a multi-dimensional Fourier analysis, showing that line defects can translate, merge, collapse and form stable singularities with even and odd parity while maintaining a stable oscillation of the spiral wave in the tissue. ...''
Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions
M. Hörning§, M. Nakahata§, P. Linke, A. Yamamoto, M. Veschgini, S. Kaufmann, Y. Takashima, A. Harada and M. Tanaka*
Scientific Reports, 7, 7760, 2017 - doi : 10.1038/s41598-017-07934-x
Brief Summary: ''... We apply a new class of revirsible rigidity switching hydrogels cross-linked by supramolecular host-guest interactions to actively control cells. In this study, the morphological dynamics of C2C12 myoblast in response to changes in substrate stiffness was observed and quantified in real time exemplarily. Actin depolarization dynamics as well as cellular responses are tracked over time. ...''