Local membrane curvature pins and guides excitable membrane waves in chemotactic and macropinocytic cells - biomedical insights from an innovative simple model M. Hörning, T. Bullmann and T. Shibata Frontiers in Cell and Developmental Biology, 9, 2467, 2021 - doi : 10.3389/fcell.2021.670943
Brief Summary: ''... Here, using 3D spatial fluctuation and phase map analysis on actin polymerization inhibited Dictyostelium cells, we reveal a spatial asymmetry of PIP3 signaling on the membrane that is mediated by the contact perimeter of the plasma membrane. We show that the contact perimeter guides PIP3 waves and acts as a pinning site of PIP3 phase singularities. The contact perimeter serves as a diffusion influencing boundary that is regulated by a cell size- and shape-dependent curvature.''
Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring J. Li, L. Zhang, L. Yu, I. Minami, S. Miyagawa, M. Hörning, J. Dong, J. Qiao, X. Qu, Y. Hua, Fujimoto, Y. Shiba, Y. Zhao, F. Tang, S. Miyagawa, Y. Chen, Y. Sawa, C. Tang, L. Liu Comunications Biology, 3, 122, 2020 - doi : 10.1038/s42003-020-0853-0
Brief Summary: ''HiPSC-derived cardiomyocytes can form three-dimensional self-organized tissue rings... the trained tissue show matured features including increased cardiac-specific gene expression, enhanced Ca2+-handling properties, an increased oxygen-consumption rate, and enhanced contractile force. A mathematical model interprets the origination, propagation, and long-term behavior of the findings.''
Mineralized scale patterns on the cell periphery of the chrysophyte Mallomonas determined by comparative 3D Cryo-FIB SEM data processing M. Hörning, A. Schertel, R. Schneider, M.-L. Lemloh, M. R. Schweikert, and I. M. Weiss Journal for Structural Biology, 209, 107403, 2020 - doi : 10.1016/j.jsb.2019.10.005
Brief Summary: ''Computational automated routines and principal component analysis of the experimentally extracted Mallomonas data created a realistic mathematical model based on the Fibonacci pattern theory. A complete in silico scale case of Mallomonas was reconstructed showing an optimized scale coverage on the cell surface.''
Precision 3D-printed cell scaffolds mimicking native tissue composition and mechanics A. Erben, M. Hörning, B. Hartmann, T. Becke, S.A. Eisler, A. Southan, S. Cranz, O. Hayden, N. Kneidinger, M. Königshoff, M. Lindner, G.E.M. Tovar, G. Burgstaller, H. Clausen-Schaumann, S. Sudhop and M. Heymann Advanced Healthcare Materials, 200918, 2020 - doi : 10.1002/adhm.202000918
Brief Summary: ''... Here, two‐photon stereolithography is adopted to print up to mm‐sized high‐precision 3D cell scaffolds at micrometer resolution with defined mechanical properties from protein‐based resins... with varying Young's moduli ranging from 7‐300 kPa are printed. This approach will allow for a systematic investigation of single‐cell and tissue dynamics.''
All computer graphics images are designed using Blender, a free and open source 3D creation suite (see www.blender.org). Some images were partly generated using Matlab and were only embeded. Here are only a few of my hobby projects shown.
This image is based on an introductory tutorial (CG Boost). I have added a red wine glass. Here, I wanted to see how I can use gravity to let rigid bodies adjust to their environment in blender.
My first project following the tutorial of Blender Guru showing my personal interpretation of a donut and a well shaped coffee mug. The latter is an important tool to work efficiently in science. ;-)
This illustration highlights my Diploma thesis. I used the materials for Enceladus and the Cassini spacecraft that are available at the NASA homepage. It's simple, but nice.
This image is supposed to highlight the mechano-sensing of motile cells on rigid substrates. My kids called it the monster cell. I build it up from skratch and used a wave-let generator in blender.
Here I tried some new features in Blender 2.80 using Fresnel effects, emission shading, and noise texture mapping.
This graphic started as a project for a potential journal cover of a paper. A similar version of that graphic got published as a homepage banner for the Biophysical Journal in 2019.
An optical mapped cardiac tissue showing Calcium waves is shown. One spiral wave is trapped on an obstacle (black circle) and one target wave originates from a pair of electrodes.
The result of a short introduction of Blender to a student, who wants to create some impressive schemes for a paper.